Prehybe DNA

Low molecular weight herring sperm, salmon sperm or calf thymus DNA is prepared by methods developed at Lofstrand Labs Limited. High molecular weight DNA is hydrolyzed by controlled alkaline digestion at reduced temperature.

The product of this reaction is a DNA molecule with an average size of 300-600 base pairs. Alkaline digestion results in DNA cleavage at non-random sites, resulting in lower network formation upon reannealing than is observed with DNA that has been sonicated or mechanically sheared.

Prehybe is ideally suited for the prehybridization of filters used in "Southern" blotting procedures. Prehybe is available in four convenient package sizes. Please indicate your required size at time of order.

Catalog # Description Unit
L3-106CT Prehybe-CTTM (Calf Thymus)  
  Pkg #1 (10 bottles/10mg each) Pkg
  Pkg #2 (4bottles/50mg each) Pkg
  Pkg #3 (1 bottle/500mg) Pkg
  Pkg #4 (1 bottle/1gm) Pkg
L3-106HS Prehybe-HSTM (Herring Sperm)  
  Pkg #1 (10 bottles/10mg each) Pkg
  Pkg #2 (4 bottles/50mg each) Pkg
  Pkg #3 (1 bottle/500mg) Pkg
  Pkg #4 (1 bottle/1gm) Pkg
L3-106SS Prehybe-SSTM (Salmon Sperm)  
  Pkg #1 (10 bottles/10mg each) Pkg
  Pkg #2 (4 bottles/50mg each) Pkg
  Pkg #3 (1 bottle/500mg) Pkg
  Pkg #4 (1 bottle/1gm) Pkg