Recombinant Library Construction & screening

A typical cloning vector.

Lofstrand Labs offers a wide range of custom molecular biology services under the heading of Custom DNA Sequencing and Molecular Cloning. Custom Library Construction, Library Screening, Library Amplification are described below in general terms with accompanying catalog numbers. Please contact one of our scientific staff for more detailed information or to receive a quote.

Lofstrand Labs provides custom PCR and RT-PCR services utilizing the Polymerase Chain Reaction process. We will also design and synthesize oligonucleotides for your custom PCR project needs.

 

Custom Recombinant Library Construction

Catalog Nr. Recombinant Library Construction Description Get A
L3-3060 Genomic Libraries (Amplified)

Turn-around time:Two to three weeks.

Quote
L3-3070 cDNA Library Construction Turn-around Time: Three to four weeks. (typical) Quote
L3-3071 Library Construction:additional 1,000,000 clones Reconstruct existing library to obtain additional clones. Quote
L3-3072 Library Amplification. Produces 1,000,000 additional clones per round. Quote
L3-3073 Library Excision, 1,000,000 clones. Includes amplification. Quote
L3-3074 Library Excision, 1,000,000 clones. No amplification. Quote
L3-3075 In vivo excision of clones. Quote

Custom Recombinant Library Screening

Catalog Nr. Recombinant Library Construction Description Get A
L3-3080-1 Library Screening 150,000 Clones. Quote
L3-3080-2 Library Screening 250,000 Clones. Quote
L3-3080-3 Library Screening 500,000 Clones. Quote
L3-3080-4 Library Screening 1,000,000 Clones. Quote
L3-3080A Plaque Lifts. Duplicate: 150,000 Clones. Quote
L3-3080B Primary Screening. 150,000 Clones Quote
L3-3080C Secondary and Tertiary Screening. Up to 5 Colonies or Plaques Quote

Lofstrand Labs will construct genomic DNA libraries in your Lambda vectors using your pre-cut vectors, packaging extracts, and genomic DNA to comply with your quality control specifications.

Quality Control (typical):
Library to contain > 106 primary clones
Titer:  approx. 106/ml (unamplified)
           >108/ml (amplified)

Lofstrand Labs will contruct cDNA libraries in Lambda or plasmid vectors using your pre-cut vectors, cDNA synthesis reagents, packaging extracts, and poly A+ RNA to comply with your quality control specifications.

Quality Control (typical):
Library to contain > 106 primary clones
Titer: approx. 106/ml (unamplified)
           >108/ml (amplified)

Hybridization services. Lofstrand also offers hybridization services for the above libraries. (Library screening does not include probe labeling reactions.) Refer to our Probe Labeling Page for details on radiolabeled and non-radioactive probes. Go There